Knock-In:Article Title: Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (M. musculus) WT C57BL/6J Jackson Laboratory Stock No: 000664 Genetic reagent (M. musculus) Rosa26-Cas9 knockin mouse Jackson Laboratory Stock No: 026179 Platt et al., 2014 Recombinant DNA reagent ER-Hoxb8-MSCV-Neo Wang et al., 2006 Recombinant DNA reagent pLentiGuide-Puro Addgene 52963 Recombinant DNA reagent lenti-sgRNA hygro Addgene 104991 Strain, strain background (M. tuberculosis) Erdman strain BEI Cat # NR-15404 Genetic reagent (M. tuberculosis) DeccC (Erdman, DeccCa1-DeccCb1) Rosenberg et al., 2015 Genetic reagent (M. tuberculosis) Lux (Erdman expressing the luxCDABEoperon) Braverman et al., 2016 Genetic reagent (M. tuberculosis) DeccC Lux Penn et al., 2018 Strain, strain background (L. monocytogenes) 10403S strain PMID: 24667708 Genetic reagent (L. monocytogenes) DactA PMID: 10931865 DP-L3078 Genetic reagent (L. monocytogenes) Dhly PMID: 7960143 DP-L2161 Genetic reagent (L. monocytogenes) DactA PlcAH86A PlcBH69G Mitchell et al., 2018 DP-L6586 Commercial assay or kit nCounter Mouse Myeloid Innate Immunity Panel NanoString .. Bone marrow derived macrophages (BMMs) were generated from the femurs and tibias from wild- type C57BL/6 (The Jackson Laboratory) mice that were 8–12 weeks old.
Recombinant:Article Title: Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (M. musculus) WT C57BL/6J Jackson Laboratory Stock No: 000664 Genetic reagent (M. musculus) Rosa26-Cas9 knockin mouse Jackson Laboratory Stock No: 026179 Platt et al., 2014 Recombinant DNA reagent ER-Hoxb8-MSCV-Neo Wang et al., 2006 Recombinant DNA reagent pLentiGuide-Puro Addgene 52963 Recombinant DNA reagent lenti-sgRNA hygro Addgene 104991 Strain, strain background (M. tuberculosis) Erdman strain BEI Cat # NR-15404 Genetic reagent (M. tuberculosis) DeccC (Erdman, DeccCa1-DeccCb1) Rosenberg et al., 2015 Genetic reagent (M. tuberculosis) Lux (Erdman expressing the luxCDABEoperon) Braverman et al., 2016 Genetic reagent (M. tuberculosis) DeccC Lux Penn et al., 2018 Strain, strain background (L. monocytogenes) 10403S strain PMID: 24667708 Genetic reagent (L. monocytogenes) DactA PMID: 10931865 DP-L3078 Genetic reagent (L. monocytogenes) Dhly PMID: 7960143 DP-L2161 Genetic reagent (L. monocytogenes) DactA PlcAH86A PlcBH69G Mitchell et al., 2018 DP-L6586 Commercial assay or kit nCounter Mouse Myeloid Innate Immunity Panel NanoString .. Bone marrow derived macrophages (BMMs) were generated from the femurs and tibias from wild- type C57BL/6 (The Jackson Laboratory) mice that were 8–12 weeks old.
Expressing:Article Title: Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond
Article Snippet: .. Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (M. musculus) WT C57BL/6J Jackson Laboratory Stock No: 000664 Genetic reagent (M. musculus) Rosa26-Cas9 knockin mouse Jackson Laboratory Stock No: 026179 Platt et al., 2014 Recombinant DNA reagent ER-Hoxb8-MSCV-Neo Wang et al., 2006 Recombinant DNA reagent pLentiGuide-Puro Addgene 52963 Recombinant DNA reagent lenti-sgRNA hygro Addgene 104991 Strain, strain background (M. tuberculosis) Erdman strain BEI Cat # NR-15404 Genetic reagent (M. tuberculosis) DeccC (Erdman, DeccCa1-DeccCb1) Rosenberg et al., 2015 Genetic reagent (M. tuberculosis) Lux (Erdman expressing the luxCDABEoperon) Braverman et al., 2016 Genetic reagent (M. tuberculosis) DeccC Lux Penn et al., 2018 Strain, strain background (L. monocytogenes) 10403S strain PMID: 24667708 Genetic reagent (L. monocytogenes) DactA PMID: 10931865 DP-L3078 Genetic reagent (L. monocytogenes) Dhly PMID: 7960143 DP-L2161 Genetic reagent (L. monocytogenes) DactA PlcAH86A PlcBH69G Mitchell et al., 2018 DP-L6586 Commercial assay or kit nCounter Mouse Myeloid Innate Immunity Panel NanoString .. Bone marrow derived macrophages (BMMs) were generated from the femurs and tibias from wild- type C57BL/6 (The Jackson Laboratory) mice that were 8–12 weeks old.
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